|
To begin a project, the customer must first
provide the nucleotide sequence information.
EMP Genetech then isolates the gene from
gene banks or amplifies it from RNA
preparations and clones the gene of interest
in appropriate eukaryotic expression vectors.
The vectors are composed of genetic
elements favorable for bacterial and
mammalian maintenance and selection,
as well as strong and stable protein
expression.
According to your guidelines, vectors for
secreted, as well as intracellular proteins
in original or tagged form, will be
constructed.
Our molecular biology competences:
-
DNA and RNA isolation
-
Plasmid preparation
-
PCR and RT-PCR Subcloning
-
Development of expression constructs and expression cells
-
Bacterial transformation and expression of fusion proteins
-
Stable transfection of human and mammalian cells
-
Assays for expression and quantification of cloned proteins
|