PRODUCTS

Product Specification Sheet

> Thrombospondin 1
> Human Alpha Thrombin
> Vitronectin
> HER2-ECD
> CTGF
> EpCAM-ECD
> mPlb2
> hPlb2
> IL6R
> EGFRvIII

Description:

EGFRvIII is a truncated isoform of the epidermal growth factor receptor (EGFR). The EGFRs (HER1-4) are a subfamily of the receptor tyrosine kinases. All family members consist of an extracellular domain, a spacer region and an intracellular domain constituting the catalytic part. The EGFRvIII gene has an in-frame deletion resulting in the deletion of amino acids 30-297 of the extracellular domain and the generation of an extra glycine at the fusion point (see aa sequence). This mutant variant is tumor specific and has not been found in normal tissues. EGFRvIII is constitutively expressed and plays a key role in tumorigenicity, invasion and therapeutic resistance. EGFRvIII preferentially activates the PI3K/Akt signaling pathway.

Source: - original full-length DNA sequence for human EGFR; aa sequence:

LEEKKGNYVV TDHGSCVRAC GADSYEMEED GVRKCKKCEG PCRKVCNGIG IGEFKDSLSI
NATNIKHFKN CTSISGDLHI LPVAFRGDSF THTPPLDPQE LDILKTVKEI TGFLLIQAWP
ENRTDLHAFE NLEIIRGRTK QHGQFSLAVV SLNITSLGLR SLKEISDGDV IISGNKNLCY
ANTINWKKLF GTSGQKTKII SNRGENSCKA TGQVCHALCS PEGCWGPEPR DCVSCRNVSR
GRECVDKCNL LEGEPREFVE NSECIQCHPE CLPQAMNITC TGRGPDNCIQ CAHYIDGPHC
VKTCPAGVMG ENNTLVWKYA DAGHVCHLCH PNCTYGCTGP GLEGCPT

- produced serum free in stable HEK293 cells
- purified from the culture supernatant

Purification:
by affinity chromatography and gel filtration

Formulation:
liquid in 20 mmol Tris, 200 mmol NaCl, pH7.0

Concentration:
200 µg/ml

Purity:
>95% reduced SDS-PAGE
MW 66 kDa

Identity:
Immuno-Blot with specific antibody

Activity:
tested in antibody binding

Stability:
at least 12 months at -70 ° C, avoid repeated freeze/thaw cycles

Sizes:
50 µg     100 µg     1000 µg

Cat.no.:
hEGFRVIII50     hEGFRVIII100     hEGFRVIII1000

Usage:
laboratory reagent - not to be used as a diagnostic product or for
administration to humans or used for any drug purposes