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PRODUCTS

Product Specification Sheet

> Thrombospondin 1
> Human Alpha Thrombin
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Recombinant Human Alpha Thrombin

Prices:

100 U
1000 U
Bulk

 

99.00 EUR
487.00 EUR
on request

Description: Thrombin is an essential component of the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin. It also activates platelets and regulates the behavior of additional cells through protease-activated receptors (PARs). Its activity is regulated by endogenous inhibitors such as anti-thrombin III or heparin cofactor. A plasma serine protease, thrombin is synthesized in the liver as a 622 amino acid prothrombin precursor with a 24 amino acid signal peptide. Cleavage by itself or by similar enzymes converts the proenzyme to three forms designated as alpha-, beta- and gamma-thrombin. Composed of a disulfide bond-linked dimer of the light chain (A) (residues 328-363) and the heavy chain (B) (residues 364-622), alpha-thrombin displays the diverse functions as described above.

Source:
- full-length DNA sequence for hn thrombin
- produced serum-free in stable HEK293 cells
- purified from the culture supernatant

aa-sequence:

TFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFR
KSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIE
KISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQA
GYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCA
GYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVF
RLKKWIQKVIDQFGE

Purification:
by heparin affinity chromatography

Reconstitution:
reconstitute in 100 µl or 1 ml H2O;

Appearance:
white powder

Concentration:
100 µg/ml

Description:
generated by activation of recombinant human prethrombin II (expressed in HEK293 cells) with ecarin.

Purity:
>95% reduced SDS-PAGE, MW 36 kDa

Activity:
tested in fibrinogen polymerisation and FXIII assays

Stability:
at least 12 months at -80 ° C, avoid repeated freeze/thaw cycles

Sizes:
100 U         1000 U

Cat.No.:
rhThr100         rhThr1000

Usage:
laboratory reagent - not to be used as a diagnostic product or for administration to humans or used for any drug purposes











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