Description: Thrombin is an essential component of the coagulation cascade in which
it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C
and thrombomodulin. It also activates platelets and regulates the behavior of additional cells through
protease-activated receptors (PARs). Its activity is regulated by endogenous inhibitors such as
anti-thrombin III or heparin cofactor. A plasma serine protease, thrombin is synthesized in the liver
as a 622 amino acid prothrombin precursor with a 24 amino acid signal peptide. Cleavage by itself
or by similar enzymes converts the proenzyme to three forms designated as alpha-, beta- and
gamma-thrombin. Composed of a disulfide bond-linked dimer of the light chain (A)
(residues 328-363) and the heavy chain (B) (residues 364-622), alpha-thrombin displays the
diverse functions as described above.
Source: - full-length DNA sequence for hn thrombin
- produced serum-free in stable HEK293 cells
- purified from the culture supernatant