PRODUCTS

Product Specification Sheet

> Thrombospondin 1
> Human Alpha Thrombin
> Vitronectin
> HER2-ECD
> CTGF
> EpCAM-ECD
> mPlb2
> hPlb2
> IL6R
> EGFRvIII

Description:

HER2/neu/ErbB-2 (human epidermal growth factor receptor 2) is a membrane glycoprotein of the ErbB family of tyrosine kinase receptors. These protein family members (ErbB1-4) serve as receptors for epidermal growth factor. ErbB2 is found on the surface of epithelial cells and is strongly overexpressed on the membrane of cancer cells especially breast cancer cells. ErbB2 has no identified ligand but rather forms heterodimers with one of the other ErbB receptors. These complexes are of high affinity to its ligands. By binding of the ligand a conformational change of the cytoplasmatic tyrosine kinase part of the ErbB2 receptor results in phosphorylation of the initial PI3K/Akt signal transduction cascade protein and effects cell proliferation. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa leader sequence, a 631 aa extracellular domain (ED), a 23 aa transmembrane region, and a 580 aa cytoplasmatic domain. The soluble ED can be shed proteolytically from the cell surface, is strongly glycosylated and of a MW of 95-105 kDa.

Source:
- DNA sequence for hn HER2-ECD (extracellular domain); aa T23-S653
- produced serum free in stable HEK293 cells
- purified from the culture supernatant

Purification:
by anion exchange chromatography

Formulation:
liquid in 20 mmol Tris, 200 mmol NaCl, pH 7,4

Concentration:
1000 µg/ml

Purity:
>95% non reduced SDS-PAGE
95-105 kDa band, partly di- and trimeric forms

Identity:
Immuno-Blot with specific antibody

Activity:
tested in Herceptin binding

Stability:
at least 12 months at -70 ° C, avoid repeated freeze/thaw cycles

Sizes:
50 µg     100 µg     1000 µg

Cat.No.:
HER2-ECD50     HER2-ECD100     HER2-ECD1000

Usage:
laboratory reagent - not to be used as a diagnostic product or for
administration to humans or used for any drug purposes